Pokročilá mikroskopie na nanoskopické úrovni
| Thesis title in Czech: | Pokročilá mikroskopie na nanoskopické úrovni |
|---|---|
| Thesis title in English: | Advanced microscopy at the nanoscale |
| Academic year of topic announcement: | 2024/2025 |
| Thesis type: | dissertation |
| Thesis language: | |
| Department: | Institute of Physics of Charles University (32-FUUK) |
| Supervisor: | Mgr. Marek Piliarik, Ph.D. |
| Author: |
| Guidelines |
| The PhD program can be integrated into the 1st Topical Partner Cluster (TPC) „Cytoskeletal Dynamics Across Scales: From molecular biophysics to organismal development“ associated with the Dresden internation Graduate School for Biomedicine and Bioengineering (DIGS-BB).
PhD student can benefit from the access to combined research infrastructure, techniques, training, lab rotations, joint summer schools. DIGS-BB TPC provides expert mentoring and thesis supervision by each student’s Thesis Advisory Committees (TAC). Each TAC is composed of three experts usually representing different disciplines and research institutions. The TAC will accompany each PhD student throughout the thesis work for maximum duration of 4 years. More information at https://www.digs-bb.de/tpc/cytoskeletal-dynamics-across-scales Detailed statement of work to be determined. More information piliarik@ufe.cz |
| References |
| H. Robert, Ł. Bujak, K. Holanová, M. Vala, V. Henrichs, Z, Lánský, M. Piliarik, Fast photothermal spatial light modulation for quantitative phase imaging at the nanoscale, Nature Communications 2021, https://doi.org/10.21203/rs.3.rs-38429/v1.
M. Vala, L. Bujak, A.G. Marin, K. Holanova, V. Henrichs, M. Braun, Z. Lansky, M. Piliarik, Nanoscopic Structural Fluctuations of Disassembling Microtubules Revealed by Label-Free Super-Resolution Microscopy, Small Methods (2021) 2000985; https://doi.org/10.1002/smtd.202000985. M. Piliarik, V. Sandoghdar, Direct optical sensing of single unlabeled proteins and super-resolution imaging of their binding sites. Nature Communications 5 (2014) 4495. S. Lin, Y. He, D. Feng, M. Piliarik, X-W Chen, Optical Fingerprint of Flat Substrate Surface and Marker-Free Lateral Displacement Detection with Angstrom-Level Precision, Phys. Rev. Lett. 129 (2022), 213201, C. F. Bohren, D. R. Huffman, Absorption and Scattering of Light by Small Particles, WILEY-VCH Verlag GmbH & Co. 2004. |
| Preliminary scope of work |
| Modern techniques of optical microscopy allow investigating light-matter interaction at the level of individual molecules. Simultaneous detection of the scattering amplitude and phase further enrich the quantitative details about the polarizability and position of nanoscopic species. Furthermore, high-speed imaging techniques have recently enabled resolving the most elusive details of the function of biological matter.
The aim of the thesis will be the development of novel ultrasensitive microscopy capable of resolving three-dimensional details of biomolecular systems at high speed. To achieve that new techniques of wavefront shaping and wavefront reconstruction will be implemented and combined with interferometric scattering microscopy. The temporal resolution of the methodology will be pushed to sub-microsecond regime to enable new modalities in label-free super-resolution microscopy based on wild-type biomolecular dynamics. |
| Preliminary scope of work in English |
| Modern techniques of optical microscopy allow investigating light-matter interaction at the level of individual molecules. Simultaneous detection of the scattering amplitude and phase further enrich the quantitative details about the polarizability and position of nanoscopic species. Furthermore, high-speed imaging techniques have recently enabled resolving the most elusive details of the function of biological matter.
The aim of the thesis will be the development of novel ultrasensitive microscopy capable of resolving three-dimensional details of biomolecular systems at high speed. To achieve that new techniques of wavefront shaping and wavefront reconstruction will be implemented and combined with interferometric scattering microscopy. The temporal resolution of the methodology will be pushed to sub-microsecond regime to enable new modalities in label-free super-resolution microscopy based on wild-type biomolecular dynamics. |