hidden - assigned and confirmed by the Study Dept.
Date of registration:
19.03.2021
Date of assignment:
19.03.2021
Confirmed by Study dept. on:
19.03.2021
Date of electronic submission:
13.03.2025
Date of proceeded defence:
29.05.2025
Opponents:
doc. RNDr. Pavel Dráber
Ing. Stanislav Vinopal, Ph.D.
Preliminary scope of work
During organismal development, growth cones at the axonal ends of neurons navigate a three dimensional maize of paths and obstacles to form the complex network of the nervous system. The cytoskeletal mechanics underpinning growth cone movements are largely unknown. Here, we will employ cultured neurons on structured surfaces to test the motion of growth cones encountering obstacles and choosing different paths. We will image cell movement through artificial maizes and correlate it with the dynamics of the intracellular actin-filament and microtubule network, with a special emphasis on the the positions and dynamics of key filament crosslinkers. This approach will allow us to determine how the cytoskeletal network drives pathfinding in response to spatial confinement of the environment.
- assigned and confirmed by the Study Dept.