Vliv solubilního endoglinu na metabolizmus žlučových kyselin v játrech u transgenních myší s vysokotukovou dietou.

• Thesis title in Czech: Vliv solubilního endoglinu na metabolizmus žlučových kyselin v játrech u transgenních myší s vysokotukovou dietou.
• Thesis title in English: Effect of soluble endoglin on bile acids metabolism in the liver of high-fat diet–fed transgenic mice.
• Academic year of topic announcement: 2019/2020
• Thesis type: rigorosum thesis
• Thesis language: čeština
• Department: Department of Biological and Medical Sciences (16-16150)
• Supervisor: prof. PharmDr. Petr Nachtigal, Ph.D.
• Author: hidden - assigned and confirmed by the Study Dept.
• Date of registration: 04.08.2020
• Date of assignment: 04.08.2020
• Confirmed by Study dept. on: 04.08.2020
• Date and time of defence: 09.10.2020 00:00
• Date of electronic submission: 01.10.2020
• Date of proceeded defence: 09.10.2020
• Opponents: MUDr. Jolana Schreiberová, Ph.D.
• Advisors: PharmDr. Eva Doleželová, Ph.D.

Guidelines

Rešerše literárních zdrojů.
Western blot.
qRT-PCR.
Statistická analýza dat.
Vyhodnocení výsledků, jejich interpretace, diskuze, závěr.

References

[1] Alsamman M, Sterzer V, Meurer SK, Sahin H, Schaeper U, Kuscuoglu D, Strnad P, Weiskirchen R, Trautwein C, Scholten D. Endoglin in human liver disiase and murine models of liver fobrosis-A protective factor against liver fibrosis. Liver Int. 2018 May;38(5):858-867
[2] Meurer SK, Alsamman M, Sahin H, Wasmuth HE, Kisseleva T, Brenner DA, Trautwein C, Weiskirchen R, Scholten D.Overexpression of endoglin modulates TGF-β1-signalling pathway in a novel immortalized mouse hepatic stellate cell line. PLoS One. 2013;8(2):e56116
[3] Kyle S. McCommis, Wesley T. Hodges, Elizabeth M. Brunt, ILKe Nalbantoglu, William G. McDonald, Christopher Holley , Hideji Fujiwara, Jean E. Schaffer, Jerry R. Colca, and Brian N. Finck Targeting the mitochondrial pyruvate Carrier attenuates fibrosis in a mouse model of nonalcoholic steatohepatitis Hepathology 2017 May;65(5):1543-1556
[4]Finnson KW, Philip A. Endoglin in liver fibrosis. J Cell Commun Signal. 2012 Mar;6(1):1-4
[5] García-Pozo L, Miquilena-Colina ME, Lozano-Rodríguez T, García-Monzón C. Endoglin: structure, biological functions and role in fibrogenesis . Rev Esp Enferm Dig. 2008 Jun;100(6):355-60
[6] García-Monzón C, Martín-Pérez E, Iacono OL, Fernández-Bermejo M, Majano PL, Apolinario A, Larrañaga E, Moreno-Otero R. Charasterization of pathogenic and prognostic factors of nonalcoholic steatohepatitis associated with obesity.J Hepatol. 2000 Nov;33(5):716-24
[7] Valbuena-Diez AC, Blanco FJ, Oujo B, Langa C, Gonzalez-Nuñez M, Llano E, Pendas AM, Díaz M, Castrillo A, Lopez-Novoa JM, Bernabeu C. Oxycholesterol-induced soluble endoglin release and its involvement in hypertension. Circulation. 2012 Nov 27;126(22):2612-24.
[8] Meurer SK, Tihaa L, Borkham-Kamphorst E, Weiskirchen R. Expression and functional analysis of endoglin in isolated liver cells and its involvement in fibrogenic Smad signalling. Cell Signal. 2011 Apr;23(4):683-99
[9] Yagmur E, Rizk M, Stanzel S, Hellerbrand C, Lammert F, Trautwein C, Wasmuth HE, Gressner AM. Elevation of endogline (CD105) concentrations in serum of patients with liver cirrhosis and carcinoma. Eur J Gastroenterol Hepatol. 2007 Sep;19(9):755-61
[10] Scherner O, Meurer SK, Tihaa L, Gressner AM, Weiskirchen R. Endoglin differentially modulates antagonistic transforming growth factor-beta1 and BMP-7 signaling. J Biol Chem. 2007 May 11;282(19):13934-43
[11] YuD, Zhuang L, Sun X,Chen J, Yao Y, Meng K,Ding Y. Particular distribution and expression pattern of endoglin (CD105) in the liver of patients with hepatocellular carcinoma. BMC Cancer 2007 Jul 4;7:122.
[12] Strasky Z, Vecerova L, Rathouska J, Slanarova M, Brcakova E, Kudlackova Z, Andrys C, Micuda S, Nachtigal P. Cholesterol effects on endoglin and its downstream pathway in ApoE/LDLR double knockout mice. Circ J. 2011;75(7):1747-55.

Preliminary scope of work

Rešerše k teoretické a metodické části
Seznámení se se základními metodikami molekulární farmakologie – Western blot, qRT-PCR
Analýza výsledků pomocí QuantityOne Software, 7500 Fast System Software
Zpracování a statistická analýza dat – GraphPad Prism Software

Preliminary scope of work in English

Review to theoretical and methodical part of diploma thesis
Getting acquainted with methods of molecular pharmacology – Western blot, qRT-PCR
Analysis of results by using QuantityOne Software and 7500 Fast System Software
Complementation of data and statistics by using GraphPad Prism Software