Understanding speciation (how species arise) is a crucial goal in evolutionary study. The reproductive isolation is a crucial step in the origin of new species and preserves a different evolutionary fate of species. My dissertation will focus on post-zygotic isolation in Munia species (Lonchura sp.) and two sister species of Nightingales (Luscinia sp.). Particularly, I will study mechanisms and genetic basis of hybrid female sterility, which are still not well understood in birds.