Příprava nové metodiky pro screening chelatorů kobaltu

• Thesis title in Czech: Příprava nové metodiky pro screening chelatorů kobaltu
• Thesis title in English: Preparation of a novel method for screeing of cobalt chelators
• Key words: kobalt, chelatace, spektrofotometrie, absorbance
• English key words: cobalt, chelation, spectrophomotometry, absorbance
• Academic year of topic announcement: 2018/2019
• Thesis type: diploma thesis
• Thesis language: čeština
• Department: Department of Pharmacology and Toxicology (16-16170)
• Supervisor: prof. PharmDr. Přemysl Mladěnka, Ph.D.
• Author: hidden - assigned by the advisor
• Date of registration: 13.09.2018
• Date of assignment: 09.10.2018
• Date and time of defence: 03.06.2020 08:00
• Date of electronic submission: 05.05.2020
• Date of proceeded defence: 03.06.2020
• Opponents: PharmDr. Jana Karlíčková, Ph.D.

Guidelines

1. nastudování dostupné literatury k tématu kobalku a jeho fyziologie a patofyziologie
2. provedení experiementů - postupné testování různých podmínek, aby bylo možné novou metodiku vyvinout a aplikovat pro patofyziologicky významné rozmezí pH (4.5-7.5)
3. zpracování výsledků
4. vlastní sepsání diplomové práce

References

Finley, B. L., K. M. Unice, B. D. Kerger, J. M. Otani, D. J. Paustenbach, D. A. Galbraith and B. E. Tvermoes (2013). "31-day study of cobalt(II) chloride ingestion in humans: pharmacokinetics and clinical effects."J Toxicol Environ Health A76(21): 1210-1224.
Fox, K. A., T. M. Phillips, J. H. Yanta and M. G. Abesamis (2016). "Fatal cobalt toxicity after total hip arthroplasty revision for fractured ceramic components."Clin Toxicol (Phila)54(9): 874-877.
Jomova, K. and M. Valko (2011). "Advances in metal-induced oxidative stress and human disease."Toxicology283(2-3): 65-87.
Leonard, S., P. M. Gannett, Y. Rojanasakul, D. Schwegler-Berry, V. Castranova, V. Vallyathan and X. Shi (1998). "Cobalt-mediated generation of reactive oxygen species and its possible mechanism."J Inorg Biochem70(3-4): 239-244.
Mao, Y., K. J. Liu, J. J. Jiang and X. Shi (1996). "Generation of reactive oxygen species by Co(II) from H2O2 in the presence of chelators in relation to DNA damage and 2'-deoxyguanosine hydroxylation."J Toxicol Environ Health47(1): 61-75.
Morrison, A. B. and J. A. Campbell (1963). "Trace Elements in Human Nutrition."Can Med Assoc J88(10): 523-527.
Paustenbach, D. J., B. E. Tvermoes, K. M. Unice, B. L. Finley and B. D. Kerger (2013). "A review of the health hazards posed by cobalt."Crit Rev Toxicol43(4): 316-362.
Shi, X., N. S. Dalal and K. S. Kasprzak (1993). "Generation of free radicals from model lipid hydroperoxides and H2O2 by Co(II) in the presence of cysteinyl and histidyl chelators."Chem Res Toxicol6(3): 277-283.
Torres-Alanis, O., L. Garza-Ocanas, M. A. Bernal and A. Pineyro-Lopez (2000). "Urinary excretion of trace elements in humans after sodium 2,3-dimercaptopropane-1-sulfonate challenge test."J Toxicol Clin Toxicol38(7): 697-700.
Tvermoes, B. E., B. L. Finley, K. M. Unice, J. M. Otani, D. J. Paustenbach and D. A. Galbraith (2013). "Cobalt whole blood concentrations in healthy adult male volunteers following two-weeks of ingesting a cobalt supplement."Food Chem Toxicol53: 432-439.