Structural studies of 14-3-3 protein complexes and their stabilization by small molecule compounds

• Thesis title in Czech: Studium struktury komplexů proteinů 14-3-3 a jejich stabilizace nízkomolekulárními látkami
• Thesis title in English: Structural studies of 14-3-3 protein complexes and their stabilization by small molecule compounds
• Key words: 14-3-3 protein, struktura, krystalografie, SAXS
• English key words: 14-3-3, protein, crystallography, SAXS
• Academic year of topic announcement: 2017/2018
• Thesis type: dissertation
• Thesis language: angličtina
• Department: Department of Physical and Macromolecular Chemistry (31-260)
• Supervisor: prof. RNDr. Tomáš Obšil, Ph.D.
• Author: hidden - assigned by the advisor
• Date of registration: 20.10.2017
• Date of assignment: 20.10.2017
• Date of electronic submission: 19.04.2021
• Date of proceeded defence: 02.06.2021
• Opponents: doc. RNDr. Jiří Brynda, CSc.
• Advisors: RNDr. Veronika Obšilová, Ph.D.

Preliminary scope of work in English

Structural studies of various complexes of 14-3-3 proteins with selected targets including peptides containing 14-3-3 binding motifs of protein kinases ASK1 and CaMKK and full-length phosphorylated proteins (CaMKK2 and others) using protein crystallography, fluorescence spectroscopy and other biophysical approaches. Project also involves preparation of recombinant proteins, presentation of obtained results at domestic and international meetings.