Preparation and characterization of Ca2+/calmodulin-dependent protein kinase kinase 2 (CaMKK2).
Thesis title in Czech: | Příprava a charakterizace Ca2+/kalmodulin-dependentní protein kinasy kinasy 2 (CaMKK2). |
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Thesis title in English: | Preparation and characterization of Ca2+/calmodulin-dependent protein kinase kinase 2 (CaMKK2). |
Key words: | protein kinasa, kalmodulin, fosforylace, protein 14-3-3, CaMKK2 |
English key words: | proteinkinase, calmodulin, phosphorylation, 14-3-3 protein, CaMKK2 |
Academic year of topic announcement: | 2015/2016 |
Thesis type: | diploma thesis |
Thesis language: | angličtina |
Department: | Department of Physical and Macromolecular Chemistry (31-260) |
Supervisor: | prof. RNDr. Tomáš Obšil, Ph.D. |
Author: | Mgr. Kateřina Mertová - assigned by the advisor |
Date of registration: | 23.10.2015 |
Date of assignment: | 23.10.2015 |
Date of electronic submission: | 06.05.2017 |
Date of proceeded defence: | 24.05.2017 |
Opponents: | prof. RNDr. Jiří Vondrášek, CSc. |
Advisors: | RNDr. Veronika Obšilová, Ph.D. |
Preliminary scope of work |
1. Vývoj expresního a purifikačního protokolu pro přípravu lidské CaMKK2. 2. Příprava mutantních forem CaMKK2 s různým počtem fosforylačních míst. 3. Fosforylace různých forem CaMKK2 a studium jejich interakce s proteinem 14-3-3 pomocí nativní ELFO a AUC. 4. Studium vztahů mezi vazbou 14-3-3 a CaM na CaMKK2. |