Strukturní charakterizace intracelulární formy myšího Nkr-p1a proteinu.
| Thesis title in Czech: |
Strukturní charakterizace intracelulární formy myšího Nkr-p1a proteinu. |
| Thesis title in English: |
Structural characterization of intracellular form of mice protein Nkr-p1a |
| Key words: |
Nkr-p1a, rekombinatní protein, chemické zesítění, hmotnostní spektrometrie |
| English key words: |
Nkr-p1a, recombinant protein, chemical cross-linking, mass spectrometry |
| Academic year of topic announcement: |
2014/2015 |
| Thesis type: |
diploma thesis |
| Thesis language: |
čeština |
| Department: |
Department of Biochemistry (31-250) |
| Supervisor: |
RNDr. Petr Novák, Ph.D. |
| Author: |
hidden - assigned by the advisor |
| Date of registration: |
19.11.2014 |
| Date of assignment: |
08.01.2015 |
| Date of electronic submission: | 10.08.2016 |
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| Date of proceeded defence: |
05.09.2016 |
| Opponents: |
doc. Ing. Petr Kolenko, Ph.D. |
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| Advisors: |
Mgr. Eliška Pospíšilová |
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| Preliminary scope of work |
| Příprava genového konstruktu (RT-PCR, cDNA), rekombinatní exprese v E. coli, in vitro sbalení, purifikace (gelová filtrace a ionexová chromatografie), elektroforesa, kovalentní modifikace proteinu |
