Testing of newly synthetized compounds with Constitutive androstane receptor (CAR)

• Thesis title in English: Testing of newly synthetized compounds with Constitutive androstane receptor (CAR)
• Key words: newly synthetized compounds, Constitutive androstane receptor (CAR), cell pharmacology; ligands
• English key words: newly synthetized compounds, Constitutive androstane receptor (CAR), cell pharmacology; ligands
• Academic year of topic announcement: 2010/2011
• Thesis type: diploma thesis
• Thesis language: angličtina
• Department: Department of Pharmacology and Toxicology (16-16170)
• Supervisor: prof. PharmDr. Petr Pávek, Ph.D.
• Author: hidden - assigned by the advisor
• Date of registration: 19.01.2012
• Date of assignment: 19.01.2012
• Date and time of defence: 05.06.2012 00:00
• Date of electronic submission: 15.05.2012
• Date of proceeded defence: 05.06.2012
• Opponents: PharmDr. Miroslav Kovařík, Ph.D.

Guidelines

Candidate should search databases for information about Constitutive androstane receptor, about induction of cytochrome P450 genes via the nuclear receptor, about ligand-binding to nuclear receptors.
In addition, candidate shoud learn cell cultivation and several molecula biology and cellular assays to test compounds with CAR.
Then candidate should perform experiments, analyze data and write diploma thesis.

References

Marino M, di Masi A, Trezza V, Pallottini V, Polticelli F, Ascenzi P. Xenosensors CAR and PXR at work: impact on statin metabolism. Curr Drug Metab. 2011 Mar;12(3):300-11.
Gao J, Xie W. Drug Metab Dispos. DecPregnane X receptor and constitutive androstane receptor at the crossroads of drug metabolism and energy metabolism.2010 ;38(12):2091-5.
Wada T, Gao J, Xie W. PXR and CAR in energy metabolism.Trends Endocrinol Metab. 2009 Aug;20(6):273-9.