Změna exprese transportních proteinů během intrahepatální cholestázy u potkanů
| Thesis title in Czech: | Změna exprese transportních proteinů během intrahepatální cholestázy u potkanů |
|---|---|
| Thesis title in English: | Alteration of transport proteins expression during intrahepatic cholestasis in rats |
| Academic year of topic announcement: | 2011/2012 |
| Thesis type: | diploma thesis |
| Thesis language: | čeština |
| Department: | Department of Biological and Medical Sciences (16-16150) |
| Supervisor: | PharmDr. Eva Doleželová, Ph.D. |
| Author: | hidden - assigned by the advisor |
| Date of registration: | 25.10.2011 |
| Date of assignment: | 02.11.2012 |
| Date and time of defence: | 06.06.2013 00:00 |
| Date of electronic submission: | 29.04.2013 |
| Date of proceeded defence: | 06.06.2013 |
| Opponents: | MUDr. Jolana Schreiberová, Ph.D. |
| Guidelines |
| Rešerše literárních zdrojů – struktura a funkce jater, transportní proteiny v játrech, intrahepatální cholestáza qRT-PCR, Western blot Zpracování výsledků Vyhodnocení a interpretace výsledků, diskuze, závěr |
| References |
| AOKI, Kimiko, et al. Effect of Aminoguanidine on Lipopolysaccharide-Induced Changes in Rat Liver Transporters and Transcription Factors. Biol. Pharm. Bull.. 2008, 31, 3, s. 412-420. DIETRICH, C. G., et al. Influence of biliary cirrhosis on the detoxification and elimination of a food derived carcinogen. Gut. 2004, 53, s. 1850-1855. CHERRINGTON, Nathan J., et al. Lipopolysaccharide-mediated regulation of hepatic transporter mRNA levels in rats. Drug Metabolism and Disposition. 2004, 32, 7, s. 731-741. HEEMSKERK, Suzanne, et al. Nitric oxide differentially regulates renal ATP-binding cassette transporters during endotoxemia. Eur J Physiol. 2007, 454, s. 321-334. HOJO, MAKOTO; SANO, NAOYO; TAKIKAWA, HAJIME. Effects of lipopolysaccharide on the biliary excretion of bile acids and organic anions in rats. Journal of Gastroenterology and Hepatology. 2003, 18, s. 815-821. KUNTZ, E.; KUNTZ, H.-D. Hepatology. Heidelberg: Springer Medizin Verlag, 2008. Morphology of the Liver, s. 16-33. LEE, JOHN; BOYER, JAMES L. Molecular Alterations in Hepatocyte Transport Mechanisms in Acquired Cholestatic Liver Disorders. SEMINARS IN LIVER DISEASE. 2000, 20, 3, s. 373-384. MESOTTEN, DIETER, et al. Growth Hormone Modulation of the Rat Hepatic Bile Transporter System in Endotoxin-Induced Cholestasis. Endocrinology. 2003, 144, 9, s. 4008-4017. RODRÍGUEZ-GARAY, Emilio Alberto . Cholestasis: human disease and experimental animal models. Annals of Hepatology. 2003, 2, 4, s. 150-158. ZINCHUK, VADIM; ZINCHUK, OLGA ; OKADA, TERUHIKO . Experimental LPS-Induced Cholestasis Alters Subcellular Distribution and Affects Colocalization of Mrp2 and Bsep Proteins: A Quantitative Colocalization Study. MICROSCOPY RESEARCH AND TECHNIQUE. 2005, 67, s. 65-70. ZOLLNER, Gernot, et al. Adaptive changes in hepatobiliary transporter expression in primary biliary cirrhosis. Journal of Hepatology. 2003, 38, s. 717-727. |
| Preliminary scope of work |
| Rešerše k teoretické a metodické části Seznámení se se základními metodikami molekulární farmakologie – Western blot, qRT-PCR Analýza výsledků pomocí QuantityOne, 7500 Fast System Software Zpracování a statistická analýza dat – GraphPad Prism Software |
| Preliminary scope of work in English |
| Review to theoretical and methodical part of diploma thesis Getting acquainted with methods of molecular pharmacology – Western blot, qRT-PCR Analysis of results by using QuantityOne Software and 7500 Fast System Software Complementation of data and statistics by using GraphPad Prism Software |