Standardizace metodiky pro in vitro stanovení chelatace mědi
| Thesis title in Czech: | Standardizace metodiky pro in vitro stanovení chelatace mědi |
|---|---|
| Thesis title in English: | Standardization of a method for in vitro assessment of copper chelation |
| Key words: | měď, chelátory mědi, metodika |
| English key words: | copper, copper chelators, methods |
| Academic year of topic announcement: | 2011/2012 |
| Thesis type: | diploma thesis |
| Thesis language: | čeština |
| Department: | Department of Pharmacology and Toxicology (16-16170) |
| Supervisor: | prof. PharmDr. Přemysl Mladěnka, Ph.D. |
| Author: | hidden - assigned by the advisor |
| Date of registration: | 21.10.2011 |
| Date of assignment: | 05.01.2012 |
| Date and time of defence: | 04.06.2013 00:00 |
| Date of electronic submission: | 22.04.2013 |
| Date of proceeded defence: | 04.06.2013 |
| Opponents: | PharmDr. Jana Ramos Mandíková, Ph.D. |
| Guidelines |
| - teoretická příprava tématu, vyhledání dostupné literatury - provedení experimentů s chelatací meďnatých i měďných iontů - statistické zpracování výsledků - příprava DP |
| References |
| Burkhead JL, Gray LW, Lutsenko S. Systems biology approach to Wilson's disease. Biometals. 2011 Jun;24(3):455-66. Collins JF, Prohaska JR, Knutson MD. Metabolic crossroads of iron and copper. Nutr Rev. 2010 Mar;68(3):133-47 Goodman VL, Brewer GJ, Merajver SD. Control of copper status for cancer therapy. Curr Cancer Drug Targets. 2005 Nov;5(7):543-9. Hashem EY, El-Zohry. SPECTROPHOTOMETRIC DETERMINATION OF COPPER(II) IN PHARMACEUTICAL, BIOLOGICAL AND WATER SAMPLES BY 4-(2′-BENZOTHIAZOLYLAZO)-SALICYLIC ACID. J App Spectr 2011,78 (4): 586-593 Lovell MA, Robertson JD, Teesdale WJ, Campbell JL, Markesbery WR. Copper, iron and zinc in Alzheimer's disease senile plaques. J Neurol Sci. 1998;158(1):47-52 Lutsenko S, Bhattacharjee A, Hubbard AL. Copper handling machinery of the brain. Metallomics. 2010 Sep 1;2(9):596-608. Maryon EB, Molloy SA, Zimnicka AM, Kaplan JH. Copper entry into human cells:progress and unanswered questions. Biometals. 2007 Jun;20(3-4):355-64. Muller P, van Bakel H, van de Sluis B, Holstege F, Wijmenga C, Klomp LW. Gene expression profiling of liver cells after copper overload in vivo and in vitro reveals new copper-regulated genes. J Biol Inorg Chem. 2007 May;12(4):495-507 Price KA, Crouch PJ, White AR. Therapeutic treatment of Alzheimer's disease using metal complexing agents. Recent Pat CNS Drug Discov. 2007 Nov;2(3):180-7 |
| Preliminary scope of work |
| - experimenty s chelatací mědi pomocí spektrofotometrického přístupu - navhované indikátory mědi ferrozin, bathocuproin a hematoxylin - studiu vlivu různých koncentrací chelátoru a různých patofyziologicky významných pH |
| Preliminary scope of work in English |
| - copper chelation experiments by use of spectrofotometric approach - proposed copper indicators ferrozine, bathocuproine and hematoxylin - effect of different concentration of indicators and different pathophysiologically relevant pH |