In situ analýzy zánětlivých kolitid Muc2-/- myší.

• Thesis title in Czech: In situ analýzy zánětlivých kolitid Muc2-/- myší.
• Thesis title in English: In situ analyses of inflammatory colitis in Muc2-/- mice
• Academic year of topic announcement: 2010/2011
• Thesis type: diploma thesis
• Thesis language: angličtina
• Department: Department of Biological and Medical Sciences (16-16150)
• Supervisor: Mgr. Marcela Vejsová, Ph.D.
• Author: hidden - assigned by the advisor
• Date of registration: 28.01.2011
• Date of assignment: 21.03.2011
• Date and time of defence: 02.06.2011 00:00
• Date of electronic submission: 02.06.2011
• Date of proceeded defence: 02.06.2011
• Opponents: PharmDr. Petr Jílek, CSc.

Guidelines

Studentka vyjela na ERASMUS
Rešerše literatury
Zvládnutí základních technik v MIVAC centru ve Švédsku
Vlastní experimenty
Zpracování výsledků
Vyhodnocení výsledků, jejich interpretace a diskuze s využitím teoretických a praktických znalostí dané problematiky

References

Muc2 protects against lethal infectious colitis by disassociating pathogenic and commensal bacteria from the colonic mucosa. Bergstrom KS, Kissoon-Singh V, Gibson DL, Ma C, Montero M, Sham HP, Ryz N, Huang T, Velcich A, Finlay BB, Chadee K, Vallance BA.
PLoS Pathog. 2010 May 13;6(5):e1000902.
Bacteria penetrate the inner mucus layer before inflammation in the dextran sulfate colitis model.
Johansson ME, Gustafsson JK, Sjöberg KE, Petersson J, Holm L, Sjövall H, Hansson GC.
PLoS One. 2010 Aug 18;5(8):e12238.
The inner of the two Muc2 mucin-dependent mucus layers in colon is devoid of bacteria. Johansson ME, Phillipson M, Petersson J, Velcich A, Holm L, Hansson GC. Proc Natl Acad Sci U S A. 2008 Sep 30;105(39):15064-9. Epub 2008 Sep 19.
Interactions between commensal intestinal bacteria and the immune system. Macpherson AJ, Harris NL. Nat Rev Immunol. 2004 Jun;4(6):478-85.
Monocyte and neutrophil recruitment during oral Salmonella infection is driven by MyD88-derived chemokines. Rydström A, Wick MJ. Eur J Immunol. 2009 Nov;39(11):3019-30.
Monocyte recruitment, activation, and function in the gut-associated lymphoid tissue during oral Salmonella infection. Rydström A, Wick MJ. J Immunol. 2007 May 1;178(9):5789-801.
Muc2-deficient mice spontaneously develop colitis, indicating that MUC2 is critical for colonic protection. Van der Sluis M, De Koning BA, De Bruijn AC, Velcich A, Meijerink JP, Van Goudoever JB, Büller HA, Dekker J, Van Seuningen I, Renes IB, Einerhand AW. Gastroenterology. 2006 Jul;131(1):117-29.
Muc2-deficient mice spontaneously develop colitis, indicating that MUC2 is critical for colonic protection. Van der Sluis M, De Koning BA, De Bruijn AC, Velcich A, Meijerink JP, Van Goudoever JB, Büller HA, Dekker J, Van Seuningen I, Renes IB, Einerhand AW.
Gastroenterology. 2006 Jul;131(1):117-29.