Vliv bakterií a mikrobiomu na dynamiku růstu mladého hostitele za podmínek chronické podvýživy a při normální dietě
| Název práce v češtině: | Vliv bakterií a mikrobiomu na dynamiku růstu mladého hostitele za podmínek chronické podvýživy a při normální dietě |
|---|---|
| Název v anglickém jazyce: | Bacteria mediated juvenile host growth promotion during chronic undernutrition and standard diet regime |
| Klíčová slova: | bezmikrobní, osa inzulin/IGF-1, somatotropin osa, probiotika, postbiotica |
| Klíčová slova anglicky: | germ-free, insulin/IGF-1 axis, somatotropic axis, probiotics, postbiotics |
| Akademický rok vypsání: | 2020/2021 |
| Typ práce: | disertační práce |
| Jazyk práce: | čeština |
| Ústav: | Katedra buněčné biologie (31-151) |
| Vedoucí / školitel: | Mgr. Martin Schwarzer, Ph.D. |
| Řešitel: | skrytý - zadáno vedoucím/školitelem |
| Datum přihlášení: | 17.10.2020 |
| Datum zadání: | 17.10.2020 |
| Předběžná náplň práce |
| Vliv bakterií a mikrobiomu na dynamiku růstu mladého hostitele za podmínek chronické podvýživy a při normální dietě |
| Předběžná náplň práce v anglickém jazyce |
| Previously, we have shown that microbiota is necessary to sustain optimal weight gain and linear growth of infant mice when fed a standard diet or a nutritionally depleted isocaloric diet (Schwarzer et al., Science 2016). Using monocolonized mouse model we showed that lactobacilli previously selected in Drosophila model of chronic undernutrition for their growth promoting capabilities were sufficient to increase linear growth of mice in a strain-dependent manner and recapitulated the whole microbiota's effect on growth. On the molecular level, we found that intestinal microbiota and Lactobacillus plantarum interact with the hormonal somatotropic axis (GH/IGF-1) activity to drive systemic growth. This exciting finding implies that there might be evolutionary conserved effects of Lactobacillus plantarum strains to promote juvenile growth. We are further dissecting the molecular mechanism behind the bacteria driven host growth promotion with the ultimate goal to improve the current treatment of undernutrition in human population. Further reading (Schwarzer et al., 2018;Schwarzer 2018;Poinsot et al., 2018). Within the framework of the Ph.D. you will master the handling of germ-free and conventional mice and work with mouse model of chronic malnutrition. Specifically, you will investigate the impact of selected probiotic, specific bacterial community, and defined isolated bacterial components on the development on the juvenile growth of weaned mice. During your PhD. study you will use classical and molecular-biological methods for bacterial detection and cultivation. You will master different methods such as ELISA, Western-blot, PCR and real-time PCR, in vitro cell and cell line cultivation, histology and immunohistochemistry. Location: Integrative Physiology of Gnotobionts group Laboratory of Gnotobiology (http://gnotobio.mbu.cas.cz/), Institute of Microbiology of the Czech Academy of Sciences, Doly 183, Nový Hrádek, 54922 Interested applicants feel free to contactschwarzer@biomed.cas.cz Integrative Physiology of Gnotobionts research group is funded by grants from Czech Grant Agency and EMBO Installation grant. |