BK-polyomavirová infekce u pacientů po kombinované transplantaci ledviny a pankreatu

• Název práce v češtině: BK-polyomavirová infekce u pacientů po kombinované transplantaci ; ledviny a pankreatu
• Název v anglickém jazyce: BK-polyomavirus infection in patients after simultaneous pancreas and kidney transplantation
• Klíčová slova: Polyomavirus BK, Kombinovaná transplantace ledviny a pankreatu, kvantitativní real-time PCR, prevalence, rizikové faktory, retransplantace
• Klíčová slova anglicky: Polyomavirus BK, simultaneous pancreas and kidney transplantation, quantitative real-time PCR, prevalence, risk factors, retransplantation
• Akademický rok vypsání: 2008/2009
• Typ práce: disertační práce
• Jazyk práce: čeština
• Ústav: externí pracoviště (11-00099)
• Vedoucí / školitel: prof. MUDr. František Saudek, DrSc.
• Řešitel: skrytý - zadáno a potvrzeno stud. odd.
• Datum přihlášení: 15.04.2009
• Datum zadání: 15.04.2009
• Datum a čas obhajoby: 15.09.2011 13:00
• Místo konání obhajoby: Ústav buněčné biologie a patologie 1. LF UK
• Datum odevzdání elektronické podoby: 18.05.2011
• Datum proběhlé obhajoby: 15.09.2011
• Oponenti: doc. RNDr. Jitka Forstová, CSc.

Seznam odborné literatury

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Předběžná náplň práce

Zavedení vyšetřovací metody BK-virémie v IKEM pro klinické použití a verifikace přesnosti této metody porovnáním výsledků laboratoře IKEM s výsledky vyšetření provedených laboratoří prof. Hirsche (Div. Molecular Diagnostics, Clinical & Transplantation Virology, Institute for Medical Microbiology, Basel, Switzerland).
Stanovení prevalence a incidence BK-virémie a BKVN v populaci diabetických příjemců po kombinované transplantaci ledviny a pankreatu (SPK) a testování rizikových faktorů replikace BK-viru v této populační skupině.
Posouzení, zda re-transplantace ledviny po selhání štěpu na podkladě BKVN je u SPK příjemců vhodným terapeutickým přístupem z hlediska možného vlivu na funkci štěpu pankreatu a zhodnocení rizika rekurence BKVN u těchto pacientů
Práce v laboratoři Langerhansových ostrůvků (laboratoř LLO PEM IKEM) zabývající se transplantacemi LLO v experimentu i v klinické praxi a na Klinice diabetologie Centra diabetologie IKEM, dále pod odborným vedením prof. Saudka a MUDr. Boučka vypracovat metodiku klinického sledování pacientů v riziku BKVN i s již prokázanou BKV infekcí, sledovat jejich laboratorní parametry a klinický stav.
V neposlední řadě pod vedením RNDr. Jarešové (Pracoviště klinické a transplantační imunologie IKEM) bude nutné provést analýzu jejich vzorků pomocí metody kvantitativní real-time PCR na přítomnost BKV DNA v séru a vyhodnocení výsledků. Výsledky takto získané porovnám s výsledky, jejichž analýzu nám laskavě provedla laboratoř Transplantační virologie, Basilej.

Předběžná náplň práce v anglickém jazyce

- Establish a specific BKV PCR protocol in our centre and to compare our results with the results analysed in the laboratory of prof. Hirsch: Div. Molecular Diagnostics, Clinical & Transplantation Virology, Institute for Medical Microbiology, Basel, Switzerland
- Assess prevalence and incidence of positive BK-viremia and BKVN in diabetic patients after simultaneous pancreas and kidney transplantation as well as to discover potential risk factors in this population
- Report whether kidney retransplantation in simultaneous pancreas and kidney transplant recipients after graft loss due to BKVN is an advisable therapeutic option with regard to sustained pancreas graft function and to risk of BKVN recurrence
- Work in the islet isolation and transplantation laboratory for their usage in experiment as well as in humans
- Establish a screening protocol of BKV infection in patients at risk as well as in patients with proven BKV infection/BKVN, therapeutic options, follow graft function parameters and clinical course
- Analyse patients samples using specific PCR protocol