Imunohistochemická detekce eNOS v aterosklerotických plátech arteria brachiocephalica

• Název práce v češtině: Imunohistochemická detekce eNOS v aterosklerotických plátech arteria brachiocephalica
• Název v anglickém jazyce: Immunohistochemical detection of eNOS in atherosclerotic lesions brachiocephalic artery
• Akademický rok vypsání: 2011/2012
• Typ práce: diplomová práce
• Jazyk práce: čeština
• Ústav: Katedra biologických a lékařských věd (16-16150)
• Vedoucí / školitel: prof. PharmDr. Petr Nachtigal, Ph.D.
• Řešitel: skrytý - zadáno vedoucím/školitelem
• Datum přihlášení: 28.11.2011
• Datum zadání: 02.11.2012
• Datum a čas obhajoby: 30.05.2013 00:00
• Datum odevzdání elektronické podoby: 08.05.2013
• Datum proběhlé obhajoby: 30.05.2013
• Oponenti: RNDr. Ivana Němečková, Ph.D.

Zásady pro vypracování

1. Rešerše odborné literatury
2. Imunohistochemická analýzy vybranných markerů ve stěně cévy
3. Praktické provedení daných úkolů
4. Zhodnocení
5. Diskuse

Seznam odborné literatury

1 L. Vecerova, Z. Strasky, J. Rathouska, M. Slanarova, E. Brcakova, S. Micuda and P. Nachtigal, 'Activation of TGF-beta Receptors and Smad Proteins by Atorvastatin is Related to Reduced Atherogenesis in ApoE/LDLR Double Knockout Mice', J Atheroscler Thromb, (2011).
2 J. F. Santibanez, M. Quintanilla and C. Bernabeu, 'TGF-beta/TGF-beta receptor system and its role in physiological and pathological conditions', Clin Sci (Lond), 121 (2011), pp. 233-51.
3 E. Perez-Gomez, G. Del Castillo, S. Juan Francisco, J. M. Lopez-Novoa, C. Bernabeu and M. Quintanilla, 'The role of the TGF-beta coreceptor endoglin in cancer', ScientificWorldJournal, 10 (2010), pp. 2367-84.
4 T. E. Walshe, V. S. Dole, A. S. Maharaj, I. S. Patten, D. D. Wagner and P. A. D'Amore, 'Inhibition of VEGF or TGF-{beta} signaling activates endothelium and increases leukocyte rolling', Arterioscler Thromb Vasc Biol, 29 (2009), pp. 1185-92.
5 H. G. Laverty, L. M. Wakefield, N. L. Occleston, S. O'Kane and M. W. Ferguson, 'TGF-beta3 and cancer: a review', Cytokine Growth Factor Rev, 20 (2009), pp. 305-17.
6 M. J. Goumans, Z. Liu and P. ten Dijke, 'TGF-beta signaling in vascular biology and dysfunction', Cell Res, 19 (2009), pp. 116-27.
7 Y. Zhai, X. Gao, Q. Wu, L. Peng, J. Lin and Z. Zuo, 'Fluvastatin decreases cardiac fibrosis possibly through regulation of TGF-beta(1)/Smad 7 expression in the spontaneously hypertensive rats', Eur J Pharmacol, 587 (2008), pp. 196-203.
8 S. Velasco, P. Alvarez-Munoz, M. Pericacho, P. T. Dijke, C. Bernabeu, J. M. Lopez-Novoa and A. Rodriguez-Barbero, 'L- and S-endoglin differentially modulate TGFbeta1 signaling mediated by ALK1 and ALK5 in L6E9 myoblasts', J Cell Sci, 121 (2008), pp. 913-9.
9 J. Rodriguez-Vita, E. Sanchez-Galan, B. Santamaria, E. Sanchez-Lopez, R. Rodrigues-Diez, L. M. Blanco-Colio, J. Egido, A. Ortiz and M. Ruiz-Ortega, 'Essential role of TGF-beta/Smad pathway on statin dependent vascular smooth muscle cell regulation', PLoS One, 3 (2008), p. e3959.
10 A. S. Maharaj, T. E. Walshe, M. Saint-Geniez, S. Venkatesha, A. E. Maldonado, N. C. Himes, K. S. Matharu, S. A. Karumanchi and P. A. D'Amore, 'VEGF and TGF-beta are required for the maintenance of the choroid plexus and ependyma', J Exp Med, 205 (2008), pp. 491-501.
11 C. L. Chen, S. S. Huang and J. S. Huang, 'Cholesterol modulates cellular TGF-beta responsiveness by altering TGF-beta binding to TGF-beta receptors', J Cell Physiol, 215 (2008), pp. 223-33.
12 J. F. Santibanez, A. Letamendia, F. Perez-Barriocanal, C. Silvestri, M. Saura, C. P. Vary, J. M. Lopez-Novoa, L. Attisano and C. Bernabeu, 'Endoglin increases eNOS expression by modulating Smad2 protein levels and Smad2-dependent TGF-beta signaling', J Cell Physiol, 210 (2007), pp. 456-68.
13 M. Ruiz-Ortega, J. Rodriguez-Vita, E. Sanchez-Lopez, G. Carvajal and J. Egido, 'TGF-beta signaling in vascular fibrosis', Cardiovasc Res, 74 (2007), pp. 196-206.
14 S. Itoh and P. ten Dijke, 'Negative regulation of TGF-beta receptor/Smad signal transduction', Curr Opin Cell Biol, 19 (2007), pp. 176-84.
15 C. L. Chen, I. H. Liu, S. J. Fliesler, X. Han, S. S. Huang and J. S. Huang, 'Cholesterol suppresses cellular TGF-beta responsiveness: implications in atherogenesis', J Cell Sci, 120 (2007), pp. 3509-21.
16 D. J. Grainger, 'TGF-beta and atherosclerosis in man', Cardiovasc Res, 74 (2007), pp. 213-22.
17 S. C. Wassmer, J. B. de Souza, C. Frere, F. J. Candal, I. Juhan-Vague and G. E. Grau, 'TGF-beta1 released from activated platelets can induce TNF-stimulated human brain endothelium apoptosis: a new mechanism for microvascular lesion during cerebral malaria', J Immunol, 176 (2006), pp. 1180-4.
18 A. Rodriguez-Barbero, J. Obreo, P. Alvarez-Munoz, A. Pandiella, C. Bernabeu and J. M. Lopez-Novoa, 'Endoglin modulation of TGF-beta1-induced collagen synthesis is dependent on ERK1/2 MAPK activation', Cell Physiol Biochem, 18 (2006), pp. 135-42.
19 M. Piao and O. Tokunaga, 'Significant expression of endoglin (CD105), TGFbeta-1 and TGFbeta R-2 in the atherosclerotic aorta: an immunohistological study', J Atheroscler Thromb, 13 (2006), pp. 82-9.
20 J. Massague and R. R. Gomis, 'The logic of TGFbeta signaling', FEBS Lett, 580 (2006), pp. 2811-20.