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Výsledky projektu Funkční 31P-magnetická rezonance pro nádorovou diagnostiku

Výsledky

▼▲Typ výsledku ▼▲Autor celku ▼▲Název celku
(Celkem 14 zázn.)
Ziółkowska, Natalia. Iron-doped calcium phytate nanoparticles as a bio-responsive contrast agent in 1H/31P magnetic resonance imaging. Scientific Reports, 2022, sv. 12, s. 2118–2118. ISSN 2045-2322. IF 4.380. [Článek v časopise]
https://doi.org/10.1038s41598-022-06125-7

We present the MR properties of a novel bio-responsive phosphorus probe doped with iron for dual proton and phosphorus magnetic resonance imaging (1H/31P-MRI), which provide simultaneously complementary information. The probes consist of non-toxic biodegradable calcium phytate (CaIP6) nanoparticles doped with different amounts of cleavable paramagnetic Fe3+ ions. Phosphorus atoms in the phytate structure delivered an efficient 31P-MR signal, with iron ions altering MR contrast for both 1H and 31P-MR. The coordinated paramagnetic Fe3+ ions broadened the 31P-MR signal spectral line due to the short T2 relaxation time, resulting in more hypointense signal. However, when Fe3+ was decomplexed from the probe, relaxation times were prolonged. As a result of iron release, intensity of 1H-MR, as well as the 31P-MR signal increase. These 1H and 31P-MR dual signals triggered by iron decomplexation may have been attributable to biochemical changes in the environment with strong iron chelators, such as bacterial siderophore (deferoxamine). Analysing MR signal alternations as a proof-of-principle on a phantom at a 4.7 T magnetic field, we found that iron presence influenced 1H and 31P signals and signal recovery via iron chelation using deferoxamine.
Ziółkowska, Natalia. Phosphorus-Containing Polymeric Zwitterion: A Pioneering Bioresponsive Probe for 31P-Magnetic Resonance Imaging. Macromolecular Bioscience, 2022, sv. e2100523, s. e2100523–e2100523. ISSN 1616-5195. IF 4.979. [Článek v časopise]
https://doi.org/10.1002/mabi.202100523

31P-magnetic resonance (MR) is an important diagnostic technique currently used for tissue metabolites assessing, but it also has great potential for visualizing the internal body structures. However, due to the low physiological level of phosphorus-containing biomolecules, precise imaging requires the administration of an exogenous probe. Herein, this work describes the synthesis and MR characterization of a pioneering metal-free 31P-MR probe based on phosphorus-containing polymeric zwitterion. The developed probe (pTMPC) is a well-defined water-soluble macromolecule characterized by a high content of naturally rare phosphorothioate groups providing a high-intensity 31P-MR signal clearly distinguishable from biological background both in vitro and in vitro. In addition, pTMPC can serve as a sensitive 31P-MR sensor of pathological conditions in vivo because it undergoes oxidation-induced structural changes in the presence of reactive oxygen species (ROS). Add to this the favorable 1H and 31P T1/T2 relaxation times and biocompatibility, pTMPC represents a conceptually new diagnostic, whose discovery opens up new possibilities in the field of 31P-MR spectroscopy and imaging.
Ziółkowska, Natalia, Presentation on "Novel phosphorous-based polymer for 31P-magnetic resonance imaging" at EMIM conference, 24-27.8.2021, Gottingen, Germany. [Jiný výsledek]
Ziółkowska, Natalia, Presentation on "Novel phosphorous-based polymer for 31P-magnetic resonance imaging" at AKTION conference, 13-15.9.2021, Pozlovice, Czech Republic. [Jiný výsledek]
Ziółkowska, Natalia, Presentation on our research group and 31P-MR experiments supported by GAUK. The presentation was prepared for PROM programme (21-27.3.2021, Łódź, Poland), during which we started the colaboration on 31P-MR spectra quantification. There was no overlap with GAUK project. [Jiný výsledek]
Ziółkowska, Natalia, Presentation on "Novel phosphorous-based polymer for 31P-magnetic resonance imaging" at ISMRM 2021 conference special X-nuclei meeting, virtual. [Jiný výsledek]
Ziółkowska, Natalia, Presentation on "Novel phosphorous-based polymer for 31P-magnetic resonance imaging" at ISMRM conference, 15-20.5.2021, virtual. [Jiný výsledek]
Vít, Martin, Presentation on Development of small animal coil for 1H/31P MR imaging. EMIM conference, virtual. [Jiný výsledek]
Ziółkowska, Natalia , Presentation on New class of biodegradable responsive phosphorus–containing contrast agent for 1H/31P MR. ISMRM conference, virtual. [Jiný výsledek]
Ziółkowska, Natalia, Presentation on New class of biodegradable responsive phosphorus–containing contrast agent for 1H/31P MR. EMIM conference, virtual. [Jiný výsledek]
Vit, Martin, Design and construction of radiofrequency coils for small laboratory animals and contrast probes at high magnetic field strength 4.7 T. [Jiný výsledek]
Ziółkowska, Natalia, Active presentation of results on Czech-Austrian Workshop on „Magnetic Resonance Imaging and Spectrosopy“ AKTION, 18 - 20.09.2019, Frein an der Mürz, Austria [Jiný výsledek]
Ziółkowska, Natalia, Active presentation of results on ESMRMB conference, 3 - 5.10.2019, Rotterdam, Netherlands [Jiný výsledek]
Vít, Martin, Vít, Martin, Active presentation of results on workshop „Pokročilé zobrazovací metody pro interdisciplinární výzkum v biologii a chemii“ 14–15. 11. 2019; Branišovice, Czech Republic. Part of the presentation is dedicated to construction of the 31P coil. [Jiný výsledek]