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Last update: BAUMRUK/MFF.CUNI.CZ (23.05.2008)
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Last update: BAUMRUK/MFF.CUNI.CZ (23.05.2008)
Students will be acquainted with the techniques of optical spectroscopy based on light scattering and their applications in physics. |
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Last update: prof. RNDr. Vladimír Baumruk, DrSc. (30.10.2019)
Oral Exam, 3 questions |
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Last update: BAUMRUK/MFF.CUNI.CZ (23.05.2008)
Harris D. C., Bertolucci M. D.: Symmetry and Spectroscopy (selected chapters), Oxford University Press, New York 1978.
Demtröder W: Laser spectroscopy (chapters 4 and 9), Springer, Berlin 1981.
Methods of Experimental Physics vol. 20: Biophysics (Ehrenstein G, and Lecar H., Eds.) (chapters 3 a 7), Academic Press, New York 1982.
Twardowski J., Anzenbacher P.: Raman and IR Spectroscopy in Biology and Biochemistry, Ellis Horwood, Chichester 1994.
Carey P. R.: Biochemical Applications of Raman and Resonance Raman Spectroscopies, Academic Press, New York 1982.
Creery R. L.: Raman Spectroscopy for Chemical Analysis, Wiley-Interscience, New York 2000.
Handbook of Vibrational Spectroscopy vol. 1-5 (Chalmers J.M., and Griffiths, Eds.), John Wiley and Sons, Chichester 2002. |
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Last update: BAUMRUK/MFF.CUNI.CZ (23.05.2008)
lecture |
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Last update: T_FUUK (14.05.2004)
Basic classification of scattering processes - elastic and inelastic light scattering. Inealstic light scattering and optical spectroscopy. 2. Quasi-Elastic (dynamic) light scattering (QELS). Correlations in time and space: relationships to frequency spectra. Dynamic theory of light scattering. Principles of experimental detection, homodyne and heterodyne methods. Aplications in biophysics, translational and rotational diffusion, polydispersity, intramolecular motion. 3. Brillouin scattering. Basic principles. Experimental techniques, Fabry-Perot interferometers. Aplications of Brillouin scattering to liquids, polymers and solids. 4. Spontaneous Raman scattering. Theoretical background, classical framework, quantum mechanical framework. Polarizability, scattering tensor, depolarization ratio. Non-resonance Raman scattering - basic properties, selection rules. Raman scattering as a technique of vibrational spectroscopy, comparison to infrared absorption spectroscopy. Resonance Raman scattering. Surface enhanced Raman scattering (SERS). Raman optical activity (ROA). 5. Modern Raman instruments and techniques. Components of Raman spectrometers- excitation lasers, spectrographs, photomultipliers, multichannel detectors. Polarized measurements in Raman spectroscopy. Sample handling, gaseous, liquid and solid samples, singl ecrystals, powders, thin films. Signal-to-noise ratio and its improvement. Difference techniques.Time-resolved Raman spectroscopy. Micro-Raman spectroscopy and Raman imaging. 6. Interpretation of Raman spectra. Molecular symmetry and selection rules in vibrational spectroscopy. Interpretation of vibrational spectra, band assignment. Structural sensitivity, conformational markers. Biophysical applications of Raman spectroscopy. 7. Nelinear techniques in Raman spectroscopy. Hyper-Raman effect. Stimulated Raman scattering and its applications. Four-photon techniques - coherent anti-Stokes Raman scattering (CARS), inverse Raman and stimulated Raman gain spectroscopy. Time-resolved CARS.
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